Regulatory

Part:BBa_K212003:Design

Designed by: Elias Scheer   Group: iGEM09_Brown   (2009-10-20)

pAgr (S. epidermidis agr operon promoter)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

On the Staphylococcal agr operon, there two promoters: P2 and P3, which are read in two different directions. Here, we have the entire promoter region. Genes may be ligated on the C terminus of this part to get the agr promoter functionality.


Source

This part was PCR'd from Staphylococcus epidermidis (ATCC 12228) genomic DNA.

References

1. Novick, Richard P., and Edward Geisinger. "Quorum sensing in staphylococci." Annual Reviews Genetics 42 (2008): 541-64. Print.